In the Prešov region there are a total of 3,094 kilometres of road.
Of all the motorway in Slovakia, only 9.14 % of it is in the Prešov region, which means that though the overall road network in the Prešov region is relatively dense, it is lacking in A-roads and motorways. Improving and completing the road infrastructure in the Prešov region, especially motorway sections to the west connecting the region with Bratislava, is therefore of great importance. A motorway connection between Poland and Hungary going through the region is also much needed.
Two of the major roads crossing the Prešov region are of international importance. These are the E 50, connecting the Czech–Slovak border with the Slovak-Ukrainian border (Drietoma – Žilina – Prešov – Košice – SR/UA border - Užhorod), and Route 371 going from Prešov – Svidník – Vyšný Komárnik – SR/PL border (Rzeszow). Another important road is Route 68, which goes up to Poland through Mníšek nad Popradom.
The Prešov region’s railway network is made up of 430 km of track and the 41 km of the Tatran Electrical Railway, with the most important railway junctions in the region in Prešov and Poprad. The Žilina – Košice line is one of the country’s major lines and is included in the AGC and AGTC system of international agreements; with gradual modernization, speeds of 120 km/h will be achieved. The Kysak – Prešov – Plaveč line forms a north-south route of international importance which is also included in the AGTC system, while the railway junction of Prešov brings together regional lines from Bardejov, Vranov nad Topľou, Humenné, Snina and Medzilaborce. Another important mode of transport in the region are public buses, providing the vast majority of journeys made by public transport.
Air connections from the region are provided by the Poprad–Tatry international airport, at 718 m above sea level the highest situated airport in central Europe. Following complete reconstruction in 1992, it provides scheduled and chartered flights, both for passengers and freight, as well as special sightseeing flights. Travellers from the Prešov region extensively use the international airport in Košice, which is situated only 47 km from the regional capital, Prešov. In Prešov itself there is a military airport in Nižná Šebastová, while in the Prešov region there are also a number of smaller aerodromes used mainly for sports purposes. These are in Zborov, Kučín, Kamenica nad Cirochou and Ražňany.
Road: Tatranská Javorina – Lysá Poľana, Mníšek nad Popradom – Piwniczna, Lysá nad Dunajcom – Niedzica, Becherov – Konieczna, Vyšný Komárnik – Barwinek
Rail: Plaveč – Muszyna, Palota - Lupków
For pedestrians and cyclists only: Nižná Polianka – Oźenna, Veľká Franková – Kacwin, Čertižné – Jaśliska, Čirč – Leluchów
Road: Ubľa – Malyj Bereznyj